Antibacterial Activity Test of Etanol Extract of Black Sea Cucumber (Holothuria atra) against the Bacteria Staphylococcus aureus and Escherichia coli
DOI:
https://doi.org/10.31965/infokes.Vol23.Iss1.1337Keywords:
Sea Cucumber, Antibacterial, Staphylococcus aureus, Escherichia coliAbstract
Black Sea cucumber (Holothuria atra) contain chemical compounds that has antibacterial activity. One of the bacteria that is pathogenic to humans and at risk of death are Staphylococcus aureus and Eschericia coli. The purpose of this study is to ascertain whether black sea cucumber ethanol extract in Pataomeme waters has antibacterial activity against the microorganisms E. coli and S. aureus. The type of research used is quantitative research with quasi laboratory experiments. Antibacterial activity test using disc diffusion method. Black Sea cucumber extract was made in 3 concentrations, consisted of 7.5%, 10% and 12.5%. The results showed that black sea cucumber extract has antibacterial activity seen through the inhibition zone formed. The maximum inhibition zone is at a concentration of 12.5% sea cucumber extract, which is 28.59 mm against S. aureus bacteria and 21.02 mm against E. coli bacteria. According to our results, sea cucumber may be a suitable marine source of antibacterial substances. To ascertain its potential use in other areas of medicine, more in vivo research must be conducted.
Downloads
References
Aba, L., & Rusliadi, R. (2020). Inventarisasi Jenis Teripang (Holothuroidea) Pada Zona Intertidal Di Perairan Pulau Ottouwe Wakatobi. Jurnal Saintifik, 6(1), 31–43. https://doi.org/10.31605/saintifik.v6i1.249
Abdulkadir, W. S., Suleman, A. R., & Hasan, H. (2021). Efek Pra Klinik Teripang Pasir (Holothuria scabra) Untuk Pemulihan Luka Insisi Pada Mencit Jantan (Mus musculus). Jambura Journal Of Chemistry, 3(2), 69–75. https://doi.org/10.34312/jambchem.v3i2.11020
Arifin, H. N., Ningsih, R., Fitrianingsih, A. A., & Hakim, A. (2013). Antibacterial activity test sea cucumber extract (Holothuria scabra) Sidayu Coast Gresik using disk diffusion method. Alchemy: Journal of Chemistry, 2(2), 101-149. https://doi.org/10.18860/al.v0i0.2882
Bordbar, S., Anwar, F., & Saari, N. (2011). High-value components and bioactives from sea cucumbers for functional foods—a review. Marine drugs, 9(10), 1761-1805. https://doi.org/10.3390/md9101761
Dwicahyani, T., Sumardianto, & Rianingsih, L. (2018). Uji Bioaktivitas Ekstrak Teripang Keling (Holothuria atra) sebagai Antibakteri Staphylococcus aureus dan Escherichia coli. World Development, 1(1), 1–15. Retrieved from: https://ejournal3.undip.ac.id/index.php/jpbhp/article/view/20384
Direktorat Jenderal Kefarmasian dan Alat Kesehatan, Kementerian Kesehatan Republik Indonesia. (2017). Farmakope Herbal Indonesia. Jakarta: Kementerian Kesehatan Republik Indonesia.
Husain, F., Yunus, F. A. M., & Basri, I. F. (2023). Aktivitas Antioksidan Dan Kandungan Total Fenolik Pada Ekstrak Teripang (Holothroidea): Antioxidant Activity And Total Phenolic Compounds Of Sea Cucumber (Holothroidea) Extract. Medical Sains: Jurnal Ilmiah Kefarmasian, 8(2), 695-704. https://doi.org/10.37874/ms.v8i2.665
Kano, N. N., Losung, F., Mangindaan, R. E., Lintang, R. A., Wullur, S., & Tumbol, R. A. (2022). Aktivitas Antibakteri Dari Teripang Laut Yang Di Peroleh Di Perairan Bunaken. Jurnal pesisir dan laut tropis, 10(1), 95-101. https://doi.org/10.35800/jplt.10.1.2022.55005
Kartikaningsih, H., A’yunin, Q., Soeprijanto, A., & Arifin, N. B. (2018). Black sea cucumber (Holothuria atra) ethanol extract as Edwardsiella tarda antibacterial. AIP Conference Proceedings, 2019 (1),1-9. https://doi.org/10.1063/1.5061919
Lee, W. T., Tan, B. K., Eng, S. A., Yuen, G. C., Chan, K. L., Sim, Y. K., Sulaiman, S. F., & Shu-Chien, A. C. (2019). Black Sea Cucumber (Holothuria atra Jaeger, 1833) rescues Pseudomonas aeruginosa-infected Caenorhabditis elegans via Reduction Of Pathogen Virulence Factors and Enhancement of Host Immunity. Food and Function, 10(9), 5759–5767. https://doi.org/10.1039/C9FO01357A
Mamangkey, E. J., Losung, F., Bara, R. A., Angmalisang, P. A., Rumampuk, N. D., & Tumbol, R. (2022). Isolasi Dan Uji Aktivitas Anti Bakteri Dari Jamur Simbion Dari Teripang (Holothuroidea sp.) Yang Diambil Di Perairan Kelurahan Molas Kecamatan Bunaken Provinsi Sulawesi Utara. Jurnal Pesisir Dan Laut Tropis, 10(2), 185–193. https://doi.org/10.35800/jplt.10.2.2022.54991
Manoppo, E. S., Wewengkang, D. S., & Kojong, N. (2017). Aktivitas Antibakteri Ekstrak Teripang Holothuria edulis Yang Diperoleh Dari Teluk Manado. Journal Pharmacon, 6(4), 44–54. Retrieved from: https://ejournal.unsrat.ac.id/v2/index.php/pharmacon/article/view/17717
Nimah, S., Ma'ruf, W. F., & Trianto, A. (2012). Uji Bioaktivitas Ekstrak Teripang Pasir (Holothuria scabra) Terhadap Bakteri Pseudomonas aeruginosa dan Bacillus cereus. Jurnal Pengolahan dan Bioteknologi Hasil Perikanan, 1(1), 9-17. Retrieved from: https://ejournal3.undip.ac.id/index.php/jpbhp/article/view/437
Oktaviani, D., Mulyani, Y., & Rochima, E. (2015). Aktivitas antioksidan dan antibakteri ekstrak jeroan teripang Holothuria atra dari perairan pulau Biawak kabupaten Indramayu. Jurnal Perikanan Kelautan, 6(2 (1), 1–6. Retrieved from: https://jurnal.unpad.ac.id/jpk/article/view/8773
Rahman, F. A., Haniastuti, T., & Utami, T. W. (2017). Skrining fitokimia dan aktivitas antibakteri ekstrak etanol daun sirsak (Annona muricata L.) pada Streptococcus mutans ATCC 35668. Majalah Kedokteran Gigi Indonesia, 3(1), 1-7.
Roihanah, S., Sukoso, S., & Andayani, S. (2012). Aktivitas Antibakteri Ekstrak Teripang Holothuria sp Terhadap Bakteri Vibrio harveyi Secara In vitro. The Journal of Experimental Life Science, 2(1), 1-5. https://doi.org/10.21776/ub.jels.2012.002.01.01
Sari, E. M., & Ma'ruf, W. F. (2014). Kajian senyawa bioaktif ekstrak teripang hitam (Holothuria edulis) basah dan kering sebagai antibakteri alami. Jurnal Pengolahan dan Bioteknologi Hasil Perikanan, 3(4), 16-24. Retrieved from: https://ejournal3.undip.ac.id/index.php/jpbhp/article/view/7771
Septiani, S., Dewi, E. N., & Wijayanti, I. (2017). Aktivitas antibakteri ekstrak lamun (Cymodocea rotundata) terhadap bakteri Staphylococcus aureus dan Escherichia coli (Antibacterial activities of seagrass extracts (Cymodocea rotundata) against Staphylococcus aureus and Escherichia coli). Saintek Perikanan: Indonesian Journal of Fisheries Science and Technology, 13(1), 1-6. https://doi.org/10.14710/ijfst.13.1.1-6
Umboh, P. M., Wewengkang, D. S., & Yamlean, P. V. Y. (2018). Aktivitas Antibakteri Fraksi Teripang Laut Holothuria atra terhadap Bakteri Escherichia coli Dan Staphylococcus aureus. Journal Pharmacon, 7(4), 88–97.
Wendersteyt, N. V., Wewengkang, D. S., & Abdullah, S. S. (2021). Uji aktivitas antimikroba dari ekstrak dan fraksi ascidian herdmania momus dari perairan Pulau Bangka Likupang terhadap pertumbuhan mikroba staphylococcus aureus, salmonella typhimurium dan candida albicans. Pharmacon, 10(1), 706-712. https://doi.org/10.35799/pha.10.2021.32758
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 JURNAL INFO KESEHATAN
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Copyright notice
Ownership of copyright
The copyright in this website and the material on this website (including without limitation the text, computer code, artwork, photographs, images, music, audio material, video material and audio-visual material on this website) is owned by JURNAL INFO KESEHATAN and its licensors.
Copyright license
JURNAL INFO KESEHATAN grants to you a worldwide non-exclusive royalty-free revocable license to:
- view this website and the material on this website on a computer or mobile device via a web browser;
- copy and store this website and the material on this website in your web browser cache memory; and
- print pages from this website for your use.
- All articles published by JURNAL INFO KESEHATAN are licensed under the Creative Commons Attribution 4.0 International License. This permits anyone to copy, redistribute, remix, transmit and adapt the work provided the original work and source is appropriately cited.
JURNAL INFO KESEHATAN does not grant you any other rights in relation to this website or the material on this website. In other words, all other rights are reserved.
For the avoidance of doubt, you must not adapt, edit, change, transform, publish, republish, distribute, redistribute, broadcast, rebroadcast or show or play in public this website or the material on this website (in any form or media) without appropriately and conspicuously citing the original work and source or JURNAL INFO KESEHATAN prior written permission.
Permissions
You may request permission to use the copyright materials on this website by writing to [email protected].
Enforcement of copyright
JURNAL INFO KESEHATAN takes the protection of its copyright very seriously.
If JURNAL INFO KESEHATAN discovers that you have used its copyright materials in contravention of the license above, JURNAL INFO KESEHATAN may bring legal proceedings against you seeking monetary damages and an injunction to stop you using those materials. You could also be ordered to pay legal costs.
If you become aware of any use of JURNAL INFO KESEHATAN copyright materials that contravenes or may contravene the license above, please report this by email to [email protected]
Infringing material
If you become aware of any material on the website that you believe infringes your or any other person's copyright, please report this by email to [email protected].
