Phytochemical Compounds of Ethanol Extract Kigelia africana (Lam.) Benth. from Pericarpium, Fructus, and Semen using LCMS/MS and in Silico Study (Potential of Phosphodiesterase-5 Inhibitor)
DOI:
https://doi.org/10.31965/infokes.Vol23.Iss4.2261Keywords:
Kigelia Africana (Lam.) Benth, Inhibitor of Phosphodiesterase type 5, PDE 5, LC-MS/MS, Molecular dockingAbstract
Erectile dysfunction can be treated by pharmacological therapy with phosphodiesterase-5 inhibitors (PDE5-I). One plant that has a function as a phosphodiesterase-5 inhibitor is Kigelia africana (Lam.) Benth. Research on Kigelia africana (Lam.) Benth. An extract as a Phosphodiesterase type 5 (PDE 5) inhibitor is still lacking. This research aims to determine the phytochemical compounds in the 70% ethanol extract of the pericarpium, fructus and semen of Kigelia africana (Lam.) Benth. with LC-MS/MS and to determine the ligand-protein interaction through in-silico studies. In previous studies, no one compared each part of the Kigelia africana (Lam.) Benth. as an aphrodisiac. The results of the identification of the 70% ethanol extract of Kigelia africana (Lam.) Benth. found several alkaloids, flavonoids, iridoids, phenolics, polyphenols, coumarins, steroids and fatty acids. Based on the results of molecular docking on 70% ethanol extract of pericarpium, fructus and semen of Kigelia africana (Lam.) Benth., 18 compounds, 20 compounds and 18 compounds were obtained sequentially, which were analyzed using LC-MS/MS. The sitosterol compound with (∆G = -8.90 kcal/mol) was identified in the three parts of the 70% ethanol extract sample of kunto bimo fruit, which showed the highest affinity for binding to the target protein compared to sildenafil with (∆G = -8.68 kcal/mol). Sitosterol compound has the same amino acid residues as the native ligand, namely ILE A:824, TYR A:664, ALA A:779, ILE A:768, ALA A:767, LEU A:765, ASN A:661, HIS A:613, PHE A:786, VAL A:782, LEU A:804, MET A:816, PHE A:820 in hydrophobic bonds. Phytochemical compounds from Kigelia africana (Lam.) Benth. It has the potential as an alternative additional therapy and a promising source for the discovery of new drugs as aphrodisiacs targeting PDE5.
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References
Agarwal, A., Makker, K., & Sharma, R. (2008). Clinical relevance of oxidative stress in male factor infertility: An update. American Journal of Reproductive Immunology, 59(2), 2–11. https://doi.org/10.1111/j.1600-0897.2007.00559.x
Agarwal, A., Nandipati, K. C., Sharma, R. K., Ritslining, C. D., & Raina, R. (2005). Role of oxidative stress in the pathophysiological mechanisms of erectile dysfunction. Journal of Andrology, 27(3), 335–347. https://doi.org/10.2164/jandrol.05136
Agyare, C., David, D. O., Yaw, D. B., & Newman, O. (2013). Anti-inflammatory and analgesic activities of African medicinal plants. In Medicinal Plant Research in Africa (pp. 725–752). https://doi.org/10.1016/B978-0-12-405927-6.00019-9
Andini, D. (2014). Potential of katuk leaf (Sauropus androgynus L. Merr) as aphrodisiac. Jurnal Majority, 3(7), 17–22.
Arifien, A. (2013). Uji efek seduhan daun katuk (Sauropus androgynus) terhadap libido tikus jantan (Rattus novergicus) dalam penggunaannya sebagai afrodisiaka dengan alat libidometer. Jurnal Ilmiah Mahasiswa Universitas Surabaya, 2(1), 1–18.
Araujo, A. B., Durante, R., Feldman, H. A., Goldstein, I., & McKinlay, J. B. (1998). Relationship between depressive symptoms and male erectile dysfunction: Cross-sectional results from the Massachusetts Male Aging Study. Psychosomatic Medicine, 60(4), 458–465. https://doi.org/10.1097/00006842-199807000-00011
Bello, I., Mustapha, W. M., Mustapha, M., Mohd, Z. A., & Roziahanim, M. (2016). Kigelia africana (Lam.) Benth. (Sausage tree): Phytochemistry and pharmacological review of a quintessential African traditional medicinal plant. Journal of Ethnopharmacology, 189, 253–276. https://doi.org/10.1016/j.jep.2016.05.049
Böhlmann, D., Onyekwelu, J. C., & Stimm, B. (2018). Kigelia africana (Lam.) Benth. Enzyklopädie der Holzgewächse: Handbuch und Atlas der Dendrologie, 1–15. https://doi.org/10.1002/9783527678518.ehg2017005
Chambers, E. E., Fountain, K. J., Smith, N., Ashraf, L., Karalliedde, J., Cowan, D., & Legido-Quigley, C. (2014). Multidimensional LC–MS/MS enables simultaneous quantification of intact human insulin and five recombinant analogs in human plasma. Analytical Chemistry, 86(1), 694–702. https://doi.org/10.1021/ac403055d
Chauhan, N. S. (2014). A review on plants used for improvement of sexual performance and virility. BioMed Research International. https://doi.org/10.1155/2014/868062
Chawla, G., & Ranjan, C. (2016). Principle, instrumentation and application of UPLC: A novel technique of liquid chromatography. Open Access Journal of Chemistry, 3(1), 1–16. https://doi.org/10.2174/1874842201603010001
Eurofins. (2022). Liquid Chromatography–Tandem Mass Spectrometry (LC–MS/MS). Eurofins. Retrieved from: https://www.eag.com/techniques/gfaas/#
Gandjar, I. G. (2007). Kimia Farmasi Analisis. Yogyakarta: Pustaka Pelajar.
Hafez, E. S. E. (2000). Reproduction in Farm Animals. USA: Lea and Febiger.
Halder, S., & Amit, S. (2017). A review on Kigelia africana. World Journal of Pharmaceutical Research, 6(11), 389–411. Retrieved from: https://www.wjpr.net/abstract_file/7905
Huang, S. A., & Lie, J. D. (2013). Phosphodiesterase-5 (PDE5) inhibitors in the management of erectile dysfunction. Pharmacy and therapeutics, 38(7), 407. Retrieved from: https://pmc.ncbi.nlm.nih.gov/articles/PMC3776492/pdf/ptj3807407.pdf
Indrisari, M. (2018). Uji efek afrodisiaka dari ekstrak bawang putih (Allium sativum) pada hewan coba mencit (Mus musculus). Akademi Farmasi Kebangsaan, 2, 140–144.
Istiqamah, F. (2024). Aktivitas afrodisiak ekstrak dan fraksi akar ginseng borneo (Rennalia elliptica Korth.) secara in silico dan in vivo. Tesis. Fakultas Farmasi Universitas Airlangga Surabaya.
Jin, L., Lagoda, G., Leite, R., Webb, R. C., & Burnett, A. L. (2008). NADPH oxidase activation: Mechanism of hypertension-associated erectile dysfunction. Journal of Sexual Medicine, 5(3), 544–551. https://doi.org/10.1111/j.1743-6109.2007.00733.x
Khairuddin, A. H. (2023). Uji aktivitas afrodisiaka seduhan kulit buah kunto bimo (Kigelia africana (Lam.) Benth.) secara in vivo. Skripsi. Fakultas Farmasi Universitas Airlangga Surabaya.
Kunwar, A., & Priyadarsini, K. (2011). Free radicals, oxidative stress and importance of antioxidants in human health. Journal of Medical Allied Sciences, 1(1), 53–60. Retrieved from: https://jmas.in/fulltext/154-1449484220.pdf?1766553077
Palanichamy, C., Parasuraman, P., Theivendren, P., Sankarganesh, A., Ewa, B., Sureshbabu, R. K. P., ... Selvaraj, K. (2022). Aphrodisiac performance of bioactive compounds from Mimosa pudica Linn.: In silico molecular docking and dynamics simulation approach. Molecules, 27(12), 3799. https://doi.org/10.3390/molecules27123799
Pratama, R. R., Sholikhah, I., Sahu, R. K., & Widyowati, R. (2023). Phytochemical compounds identification from 70% ethanol extract of Arcangelesia flava (L.) Merr stems using LC-MS/MS and in-silico molecular docking approach as inhibitor interleukin-1β. Pharmacognosy Journal, 15(4). https://doi.org/10.5530/pj.2023.15.114
Putri, B. E. (2014). Kadar polifenol dan efek antioksidan ekstrak etanol buah sosis (Kigelia africana (Linn.) Benth.) serta aplikasinya dalam sabun transparan. Skripsi. Universitas Kristen Satya Wacana.
Ritchie, C., & Ko, E. Y. (2021). Oxidative stress in the pathophysiology of male infertility. Andrologia, 53(1), e13581. https://doi.org/10.1111/and.13581
Roy, K., Kar, S., & Das, R. N. (2015). Understanding the basics of QSAR for applications in pharmaceutical sciences and risk assessment. https://doi.org/10.1016/B978-0-12-801505-6.00010-7
Rusdi, N. K., Ni Putu, E. H., & Maifitrianti, Y. S. U. (2018). Aktivitas afrodisiaka fraksi dari ekstrak etanol 70% daun katuk (Sauropus androgynus (L.) Merr) pada tikus putih jantan. Pharmaceutical Sciences and Research, 5(3), 123–132. https://doi.org/10.7454/psr.v5i3.4100
Sikka, S. C. (2001). Relative impact of oxidative stress on male reproductive function. Current Medicinal Chemistry, 8(7), 851–862. https://doi.org/10.2174/0929867013373039
Sikka, S. C., Rajasekaran, M., & Hellstrom, W. J. (1995). Role of oxidative stress and antioxidants in male infertility. Journal of andrology, 16, 464-468. https://doi.org/10.1002/j.1939-4640.1995.tb00566.x
Wahdaningsih, H. S., Sartika, D., & Fajriaty, I. (2012). Uji Aktivitas Afrodisiaka Ekstrak Etanol 70% Daun Tapak Liman pada Mencit Putih Jantan Galur BALB/C. Skripsi. Pontianak: Fakultas Kedokteran, Universitas Tanjungpura.
Wardani, I. G. A. A. K., & Santoso, P. (2017). Efektivitas afrodisiaka dari ekstrak etanol jahe merah (Zingiber officinale Roscoe) pada tikus (Rattus norvegicus L.) putih jantan. Jurnal Ilmiah Medicamento, 3(1), 22–28. https://doi.org/10.36733/medicamento.v3i1.1045
Yafi, F. A., Jenkins, L., Albersen, M., Corona, G., Isidori, A. M., Goldfarb, S., ... & Hellstrom, W. J. (2016). Erectile dysfunction. Nature reviews Disease primers, 2(1), 1-20. https://doi.org/10.1038/nrdp.2016.3
Zulkarnain, S., Sijid, S. A., Amrullah, S. H., & Rukmana, R. (2022). Keanekaragaman tanaman berpotensi sebagai afrodisiak alami. Teknosains: Media Informasi Sains dan Teknologi, 16(2), 255–260. https://doi.org/10.24252/teknosains.v16i2.28752
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